Journal: Macromolecular Bioscience

Article Title: Identification of Novel Growth Factor Conjugated Nanofibers for Stimulation of Neuronal Growth

doi: 10.1002/mabi.202500585

Figure Lengend Snippet: SAPs stimulate the growth of primary mouse neurons. Mouse hippocampal neurons were plated on coverslips without coating (A), coated with SAPs1‐9 (BJ), or PLL/lam (K). After 24 h in culture, neurons were stained for βIII tubulin (green), Phalloidin (red) to label F‐actin, and DAPI (blue). Inserts reveal higher magnifications of dashed boxes. (A) Uncoated coverslips (blank) did not provide a growth substrate for neurons. (B‐J) Coating coverslips with the different SAPs resulted in neuronal growth to a variable extent (quantified in L). (K) PLL and laminin were employed as a favorable growth substrate, resulting in neuronal growth. (L) Quantification of neuron numbers/area according to three different neuron sizes (small, medium, large). N‐numbers included 3 separate experiments consisting of 3–6 pooled animals per experiment. For statistical analysis, Kruskal‐Wallis and Dunn's multiple comparisons test were performed. All significant changes were indicated; otherwise, no significant changes were obtained. Significance was calculated in relation to blank ( * , ** , *** reflecting P ≤ 0.05, 0.01, and 0.001, respectively). Scale‐bar (A–J) = 100 µm; (inserts) = 50 µm.

Article Snippet: Cells were washed 3 times with PBS and then incubated for 1 h in the dark at room temperature with Alexa Fluor 488 donkey anti‐rabbit (2 μg/mL, A21206, Thermo Fisher) and Phalloidin Texas Red (1U/mL, #00033, Biotium).

Techniques: Staining